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These functional and structural changes are associated with endothelial cell (EC) and vascular smooth muscle cell (VSMC) dysfunction, infiltration of immune cells in arterial tissues, and alterations in the extracellular matrix (ECM) of arteries
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Many drugs enter the BBB primarily through the transcellular pathway, so we can determine the physiological functional integrity of the in vitro model by immunofluorescence techniques to detect the amount of several membrane inward and outward transport proteins, such as GLUT-1, the transferrin receptor or P-glycoprotein[208]
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