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Since CBS induces the expression of cystine importer SLC7A11 in HK-2 cells (Figure 3D), we further supplied the cells with 0.1 mg/ml of cystine in the culture medium, which dramatically increased the absorption of bismuth (Figure 5A)
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Fmoc-AA-OH (5 eq.), HBTU (4.8 eq.), and DIPEA (10 eq.) were dissolved in DMF and agitated with the resin for 1 h
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