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Purification of glutathione S-transferase enzyme from liver tissue of shabout (Barbus grypus Heckel) and investigation of the inhibition effect of some metal ions under in vitro conditions | Fish Physiology and Biochemistry
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The cells were harvested by centrifugation at 6000 rpm for 30 min at 4 °C, followed by snap-freezing in liquid nitrogen and storage at −80 °C
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