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The activity of efflux pumps depends largely on PMF [10] and PMF levels were significantly increased in the NAC group compared with the control group (Figure 5c)

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The column was then washed with 10 CV of 25 mM imidazole (Sigma-Aldrich) in DPBS (pH 7.2), followed by elution with 10 CV of 250 mM imidazole in DPBS (pH 7.3)