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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 μg peptides was transferred to a new low-bind Eppendorf tube per sample
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4.1.2 HPA axis dysfunction and PTSD Though the HPA axis maintains the body’s endocrine balance by precise negative feedback mechanisms, dysfunction in this system can lead to severe physiological and psychological problems (Yehuda, 2001)
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