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Maca: Benefits, Side Effects, Safety Tips, Dosage, and More

PLP Protonation State and Reaction Specificity Schiff bases of pyridoxal and its derivatives can exist in several different protonation states that differ in their intrinsic reactivity toward carbanion formation.[39, 42, 47] Limbach and coworkers, in collaboration with our group, have used N-15 NMR of pyridine nitrogen-labeled PLP to understand the factors that control protonation states of free and AAT-bound PLP.[48–55] Initially, model studies were performed with 3,5-dibromosalicylaldehyde imines in solvents of varying polarity.[55] It was found that more polar solvents promoted the proton transfer from the phenolic oxygen to the imine nitrogen, as expected for formation of a dipolar ionic species from a neutral one

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