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2 Materials and methods 2.1 Identification of ABC transporters in blackgrass To identify potential transporter genes encoding ABC and multidrug and toxic compound extrusion (MATE) proteins, contig sequences from the NTSR blackgrass transcriptome were assembled ( using the online protein Blast (BlastP) analysis tool ( The sequences of ABCC transporters were confirmed by amplifying the respective open reading frames (ORFs) by polymerase chain reaction (PCR) using cDNA prepared from NTSR blackgrass using specific primers from untranslated regions (UTR)
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