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Subsequently, slides were washed 3X with PBS (10 min, RT) and incubated with the secondary horseradish peroxidase conjugated antibody (mouse anti-rabbit IgG, 0.4 mg/mL diluted 1:500, sc-2357 or goat anti-mouse IgG, 0.4 mg/mL diluted 1:500, sc-2031) for 2 h at RT, followed by three PBS washes and treated with the 3,3′-diaminobenzidine substrate (ab64238, Abcam) for 10 min
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[DOI] [PMC free article] [PubMed] [Google Scholar] 68.Mohamed A.Z., Andersen T., Radovic S., Del Fante P., Kwiatek R., Calhoun V., Bhuta S., Hermens D.F., Lagopoulos J., Shan Z.Y
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