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Cultured cells were stimulated for 6 h with PMA (50 ng ml − 1 ) (BioGems) and ionomycin (1 µg ml − 1 ) (BioGems) in the presence of brefeldin A (1 μg ml − 1 ) (GolgiPlug, BD Biosciences), stained for viability (Zombie UV Fixable Viability Dye (BioLegend)) and surface markers, fixed and permeabilized with 3.7% formaldehyde (Sigma) and 0.1% NP-40, respectively, and stained for intracellular cytokines

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