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Research applications Transwell and scratch migration assays with fibroblasts, keratinocytes and endothelial cells Endothelial tube formation on basement membrane matrix, with VEGFR2 knockdown or kinase inhibitor controls Western blotting and phospho-specific assays for FAK, paxillin, Akt and eNOS Nitric oxide measurement by Griess assay or NO-sensitive fluorescent probes in endothelial cultures qPCR and reporter assays for Egr-1, NAB2 and growth hormone receptor expression Stability and RP-HPLC method development in acidic buffers, using the peptide as a proline-rich, aromatic-free test analyte BPC-157 sits in the Tissue Repair Signalling category with the other peptides used in cell migration and extracellular matrix work