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For staining of activated endoneurial macrophages 47,48,49 , fresh-frozen tibial nerve samples were fixed in acetone, blocked for 30 min with 5% bovine serum albumin (BSA) in 0.1 M PBS, followed by incubation overnight with mouse anti-rat ED1 antibodies (1:500, MAK0341R, Linaris) in 1% BSA in 0.1 M PBS at 4 °C
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