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MM-GBSA of few conformers based on their binding affinity and cluster size was done using Prime module 60 of the Maestro

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Experiments have been carried out using the cDNA of IGF-1(a compound which was responsible for directing over expression of IGF-1) in an adenoviral vector under the control of a muscle regulatory sequence. Results were shown by Fig.1. It was found that a single intramuscular injection of IGF-1 cDNA into a mouse muscle resulted in a 15% increase in mean muscle fibre cross section area and a 14% increase in strength in young adult mice within four months. It was concluded due to the anabolic effect of IGF-1. But those effects are not all that the researchers have found. IGF also seems to prevent aging-related muscle changes in old adult mice. These old mice experienced a 27% increase in strength as compared with uninjected old muscles. Muscle mass and fiber type distributions were maintained at levels similar to those in young adults. The researchers have speculated that these effects are primarily due to stimulation of muscle regeneration via the activation of satellite cells by IGF-I