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The number of astrocytes is not significantly increased until 3 weeks of cuprizone treatment, simultaneously with first signs of apparent demyelination

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For FA, the continuous increase in the RD may have a main and greater impact on FA, and such factors like reduction of myelin sheath integrity and fiber density, and edema may ultimately have led to the destruction of microscopic integrity of tissues 37,38 , which was manifested as a continuous decrease in FA from the 4w group to the 6w group 22