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Briefly, the assay reagent, based on the LAL, was reconstituted and applied to 50 μL of samples and LPS standards in a 96-well plate

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Non-negotiable targets: Protein: 80-100g daily minimum (prevents muscle loss during weight loss) Calories: Don’t go below 1,200 (women) or 1,500 (men) despite suppressed appetite Fiber: 25-35g daily (prevents constipation, supports gut health) Healthy fats: 30-50g daily (prevents gallbladder issues during rapid weight loss) Meal structure moving forward: Still smaller frequent meals (5-6 per day)