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The inhibitory strength was expressed as IC 50 value, which is the concentration of the inhibitor needed to inhibit half of the enzyme activity in the tested condition
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The persistent actions of norBNI on KOR-agonist mediated analgesia (21 days) were shown to require JNK, as JNK1 isoform knockout mice show an absence of norbinaltorphimine-dependent 21 day KOR blockade, and selective JNK inhibitors prevented the long-lasting norBNI effect
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