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250 ⁇ l of PBS containing human HGF (1.25 ng) were incubated with 3 H-Hinge, the central dimerization domain of HGF, in the presence of varying concentrations of 6-AH analogs between 10 ⁇ 13 M to 10 ⁇ 7 M (half-log dilutions) for 40 min at 37° C
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