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The proteins were subject to sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using Bolt 4โ12% Bis-Tris gel (ThermoFisher, Eugene, USA) at 80 V for 30 min and then 120 V for 60 min with PageRulerโข Prestained Protein Ladder or SeeBlue Prestained Protein Standard (Invitrogen #LC5625) (ThermoFisher, Eugene, USA)
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J Physiol Pharmacol 2011;62(3):327-34
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