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After treatment of 35 mg oral trimetazidine twice daily for 12 weeks, the oxidative stress markers, including malondialdehyde and 8‐oxo‐dG, and the resting energy expenditure were reduced in patients with amyotrophic lateral sclerosis, which may affect the disease progression of amyotrophic lateral sclerosis [199]

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After three washes with PBS containing 0.5% Tween-20, the membranes were incubated with a diluted horseradish peroxidase-conjugated secondary anti-rabbit (GE Healthcare, Buckinghamshire, UK) at room temperature for 2 h